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The reason for this result remains unclear but might be explained if the B-repeats not only bind Fg but also bind a second ligand implicated in virulence, causing attenuation of mutants even in Fg-deficient mice.
These results strongly indicate that a global stress response has been triggered in the three DnaJ mutants even in the absence of external stress.
Examination of the toeprint gels shows visible toeprints at the pretranslocation position in the delta 1 and delta 2 mutants, even in the absence of the antibiotic.
Our data showed that target gene restoration (TGR) occurred in IDM mutants, even in the presence of antibiotics, while the IFD mutants were stable.
Consequently, use of sulfonamides is widespread in Paris and Lyon, favoring the emergence of mutants and provoking a high risk for incidental acquisition of these mutants, even in patients not directly exposed to sulfonamides.
Notch inhibition in vhl mutants without blood flow, either by morpholino knockdown of dll4 or pharmacological inhibition with gamma secretase inhibitors leads to embryonic death, which has prevented us from testing the hypothesis that Notch inhibition might rescue the excessive angiogenesis in vhl mutants even in the absence of blood flow.
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Similar experiments were performed with whole plants instead of leaf disks: again, an increased bleaching and MDA production was observed in mutants, even if, due to the unavailability of a large 4°C growth chamber, high light treatments were performed at 22°C for a longer time (4 days) in order to obtain comparable MDA productions.
The absence of GMP synthesis would then prevent the growth of the hprT mutant even in LB medium.
Therefore, Nhp10 gene-specific binding is disrupted in the H3K37A mutant even in the context of normal TORC1 signaling whereas Hmo1 binding is not significantly impeded.
Instead, Tor inactivation also required loss of a positive nutrient signal to trigger autophagy and loss of both was sufficient to activate autophagy in the buffy mutant even in the presence of enforced phosphoinositide 3-kinase (PI3K) signaling.
It should be noted that the isolated proteins (ALKBH4 and mutants), even when kept in iced-cooled solutions (4 °C), aggregated and then precipitated at concentrations higher than 0.5 mM.
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