As shown in Figure 3B, the activity of the double mutant was not markedly less than either single DPE mutant, either in HeLa or HeLa/CIITA cells.
Morphological inspection of single mutant (either in Col or L er backgrounds) and double-mutant (Col background) plants was done with plants grown in the greenhouse under regular growth conditions.
In budding yeast, mutants of the adenine biosynthesis pathway are long-lived (Matecic et al. 2010), whereas the corresponding mutants either decrease in abundance or are not detected in our screen (Table S2).
In contrast, in the mutants, either directly or as consequence of a facilitated putative M213 oxidation as for V210I and R208H, the interacting regions change.
Six of the 55 mutants either grew better or worse in vivo than in vitro, respectively.
We examined glomerular fusion in vangl2 MO-injected embryos and in tri/vangl2 mutants, either with or without injection of ofd1 MO (Fig. 6P T and data not shown).
Two genes where cloning and sequencing provided explanations for their complexity are rudimentary, where complementing mutants affect distinct domains in the protein [18] and cut, where complementing mutants map either in the regulatory region or in the coding exons of the gene [19].
beta-Arrestin 1 mutants, impaired either in c-Src binding or in the ability to target receptors to clathrin-coated pits, acted as dominant negative inhibitors of beta2 adrenergic receptor-mediated activation of the MAP kinases Erk1 and Erk2.
