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Specific cellulase activity was slightly higher in all three photoreceptor mutants early in utilization of cellulose compared to WT (28 and 40 hrs), but the Δ vvd mutant showed significantly more cellulase activity after 5 days of growth on Avicel.
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In addition, the fact that mutants earlier in the pathway have not been identified implies that a complete loss of sterol might be lethal to the alga under normal growth conditions.
Similar to that observed in the β-catenin LOF mutants, early TM treatment resulted in hypoplasia during URS/perineal formation in the β-catenin GOF mutants.
This gene is also named EFS as it was identified as the one affected in the mutant early flowering in short days (efs) in the Landsberg erecta (Ler) ecotype, and mutations also result in earlier flowering in the Colombia (Col) ecotype (Table 1) [7], [7].
In these three mutants early NF signalling and cortical cell activation are not seriously modified [32], [86] [88].
Since there is relatively little correlation between the number and average time of mutants produced from the sensitive cell population, this indicates that late recurrence occurs due to the death of resistant mutants produced early in the temporal history of treatment.
We were unable to test DDX-17::GFP for cell-autonomous function and relevance of its subcellular localization since this RBP was identified from RNAi, and ddx-17 mutants die early in development.
The simplest interpretation is that translational derepression of cdc-25.3 contributes to premature M-phase entry of lin-41 mutant oocytes early in their development.
Despite the altered chromatin structure in mutant early blastocysts, Cdx2 expression remained restricted to the TE and was not detected in EPI cells (data not shown), which is consistent with previous reports [48].
The flm jmj13 and svp jmj13 double mutants flowered earlier in all conditions, including 22 °C and SD, whereas jmj13 showed a wild-type flowering phenotype in 22 °C and SD (Supplementary Fig. 8a).
Since one of the possible effects derived from this extended lapse of time could be the development of additional TOs, we looked for the existence of cells with MTOs in several gliding mutants isolated earlier in our laboratory [17].
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