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The team can take any one microbe and create a library of thousands of mutants, each of which contains a broken copy of a single gene.
Our results obtained with either point mutations or insertion or deletion mutants (each of which rotated the helix structure at the CRP binding site one-half turn) showed significant decrease of penicillin amidase (PA) activity, suggesting the CRP as a major activator.
In order to identify more genes involved in memory formation, we previously generated 2,667 enhancer trap mutants, each of which contains a p-element (P{GawB}) insertion (Liu et al., 2008).
However, in contrast to the between-dimer mutants, each of these within-dimer mutants completely failed to disassemble pre-formed wild type polymers.
Pools of 25 mutants each of which harbouring different signature tags were instilled into murine airways and bacteria were recovered 48 hours later from lungs, spleen and liver.
Using PB-mediated germline mutagenesis, we generated mouse mutants, each of which carries a single PB transposon insertion (Ding et al., 2005) (S.D., X.W. and T.X., unpublished data).
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We utilized either the SLX4L1022A or the TRF2F120a mutant, each of which can independently abolish the SLX4-TRF2 interandindividuallyividually expressed each endonuclease with SLX4L1022a or TRF2F120a (data not shown) and assayed their nuclear localization.
To corroborate the effects of mod-1 and ser-6 on FLP-7mCherry secretion with changes in body fat content, we generated double mutants between each of the receptor mutants and the flp-7 peptide mutant.
We also prepared double mutants for each of FABP5 (R109and129A) and FABP7 (R107asR127A), as binding pocket-destructive mutants [negative controls for 1-anilinonaphthalene-8-sulfonic acid (ANS) titration assay].
Therefore, we genetically engineered virus mutants each containing one of these 3 substitutions; they all exhibited HA-deficiency.
Null mutants of each of the genes are viable, and have phenotypes similar to those of the point mutants.
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