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This enabled us to observe the differential decrease of residual activity among mutants due to structural destabilization during the heat treatment processes.
Increased expression of multiple LC3 isoforms are observed in Atg7 mutants due to impaired control of the two ubiquitin-like conjugation systems of LC3 and Atg12 (Thompson et al., 2005).
The reason for this phenomenon was speculated to be improved growth of all mutants due to their enhanced tolerance to ethanol stress, which was verified through higher mutants of cell density of R-M12C4-P3 R-M12C4-P3 R-M12C4-P3recorded yield of 2.07 g/L (DCW) compand R-M18C4-P3 R-M18C4-P3 R-M18C4-P3(DCwhich
It is also possible that we did not detect a phenotype in Frizzled mutants due to functional redundancy of these receptors.
They are almost never observed when only gcm is mutant, perhaps because a few glia are still present in these mutants due to Gcm2 expression.
In addition, esk1-5 aba3-1 double mutants were smaller than both dwarf single mutants due to the combined effects of ABA deficiency and reduced water transport.
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Kinetic analysis shows that the E197S mutant is considerably more active than the corresponding alanine mutant due to a 40-fold increase in kcat.
The photosynthetic efficiency was also improved in the roc5 out1) mutant due to an increase in the stomatal conductance and photosynthetic rate (Zou et al. 2011).
This hypothesis is highly unlikely with the RPE65 R91W mutant due to its extremely low level of expression compared to the wild type protein (Fig S1C, [27]).
In the context of our biochemical results, the simplest explanation is that active Wnt1 is secreted prematurely in the mutant due to decreased Oto-mediated ER retention.
While methodological differences might account for some of the variation we also cannot exclude polar effects in the sap1 3Δ mutant due to the use of the URA-blaster method in mutant generation.
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