Exact(2)
Using this assay, we measured apparent KM and kcat values for DAAT and identified mutants displaying altered substrate specificity via the screening of cell lysates in 96-well plates.
In the following, we explore and validate this approach on three biologically relevant examples: (1) the quantitative comparison between wild-type and mutants displaying altered locomotion (Fig. 3, Fig. 4), (2) the effects of aging on the locomotory rate of worms (Fig. 3) and (3) the actual separation of a mix of two worm strains (Fig. 4, Fig. 5).
Similar(58)
All the charge mutants displayed altered titration behaviour of active site electrostatic potentials.
dnrx and dnlg1 single and double mutants display altered subcellular distribution of Discs large (Dlg), which is the homolog of mammalian post-synaptic density protein, PSD95.
Additionally, mutants displayed altered emotional responses as indicated by decreased freezing in contextual and cued fear conditioning and reduced fear-potentiated startle.
These mutants displayed altered kinetic characteristics that may explain their defects in catalysis.
hos9 and sfr6 mutants display altered sensitivities to freezing stress without affecting CBF/DREB1 gene expression [47], [48], as also seen in lov1 mutants.
In A. nidulans, barA1 mutants display altered pattern of filipin staining, suggesting a defect in organizing sterol-rich domains at the hyphal tip [15].
The picup1 and twirt1 (twr-1) mutants display altered root meristem function resulting in short roots, with twr-1 mutants also affecting the shoot meristem.
Furthermore, regarding auxin signalling, the sar3 and double sar1 sar3 mutants display altered localization of AXR3/IAA17, which is an auxin response repressor from the AUX/IAA family.
Both mutants displayed altered vegetative growth phenotypes with rpsJ-S32A showing slower growth kinetics and rpsJ-S32D exhibiting an extended lag period (Fig. 5d).
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