Only unc-112 mutants displayed lowered maximal ATP production when pyruvate + malate and succinate were used as substrates (P < 0.05).
We observed that dbh mutants display lower overall activity and activity when awake during both day and night.
The hsi2 mutant displayed lower constitutive and ABA-induced stomatal conductance than wild-type and accumulated lower levels of ABA metabolites and several osmolytes and osmoprotectants following water withdrawal.
In addition, the Trp mutant displayed lowered substrate inhibition for both natural and artificial substrates, while Km of substrates increased and thermal stability at 50 °C decreased ∼100-fold.
With urea, Arg or Orn as the main nitrogen source, free Gln levels were progressively lowered and the oat mutants always displayed lower Gln content than the wildtype, although differences were only significant on 5 mM Arg (Fig. 5C,D and Fig. 6).
Most mutants showed comparable or somewhat higher protein levels than WT IRAK-M upon stable expression in THP-1 and H292 cells, however the hyperactive mutant R70Q displayed lower expression, and the D19N-A23S mutant displayed markedly lower steady state protein levels.
N84A and R85A mutant proteins displayed lower heme binding affinities, consistent with the finding that Asn84 interacts with propionate of protoporphyrin IX, and Arg85 with Asp40 on the heme proximal side.
Consistent with a role for YeaG in allowing bacteria to adapt to adverse growth environments, a comparative analysis of virulence carried out with a S. Typhimurium ∆yeaGH and wild-type strains indicated that mutant bacteria displayed lower proliferation in the spleen (Fig. 6C), thus implying that yeaG could be a determinant the overall fitness of S. Typhimurium.
The EC50 of the single mutant that displayed lower than WT activity, Y808W, was also unchanged.
Moreover, mutant glands displayed lower expression levels of ribosomal RNA and proteins as well as a general decrease in translation.
