It is known, that the shape of pavement cells and the extension of lobes, depends also on the microfilament dynamics and that most of the distorted mutants display less lobed morphology of pavement cells [ 3, 34, 59].
The ΔF4 mutant displayed less enzyme activity than R-BSX.
All strains tested were sensitive to Mn2+ however, only the hsp104 mutant displayed less sensitivity than the wild type (48% versus 37%; Figure 2(b), black bar).
The Orai1 E106D pore mutant displayed less Ca2+-selectivity together with a left-shifted reversal potential and an enhanced pore diameter [59 61].
In contrast, the lysates containing transfected interface mutants or Bax displayed less than 15% of the DEVD-ase activity of the WT lysates, despite the high amounts of Annexin V-positive cells present in the cell culture.
In contrast, cells overexpressing the negative cytosolic mutant GFP-Rab14ΔGCGC displayed less red fluorescence intensity associated with the inclusions compared to GFP-Rab14wt overexpressing cells (Figure 8B).
Far-UV spectroscopy showed that three of these mutants exhibited similar secondary structure contents as WT CXCL8, whereas two mutants differed significantly: CXCL8 Δ6F17RF21RN71K) displayed less than 50% of the helical and more than 150% of the CXCL8 sheet content, and CXCL8 Δ6F17RF21RE70KN71R) exhibited more than 200% of the helical but similar sheet content compared with CXCL8.
Furthermore, a BAR domain mutant of Pinkbar (W141S) that is less efficient in forming oligomers (Pykäläinen et al., 2011) displayed less pronounced effects on phosphoinositide dynamics as compared to the wild-type BAR domain of Pinkbar.
When plants were given a root-drench treatment with ABA (30 μM) or PBI425 (20 μM) before withholding water, the onset of visible wilting was delayed in all genotypes by three to four days (until 7 – 10 days after treatment), after which the mutant continued to display less wilting, and OEx1 more wilting, than the wild-type at all observation times.
