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The R715A, Q829A, and R675A mutants displayed elevated Michaelis constants for MgADP in the partial back reaction.
Consistent with the yeast and human cell data, Ago mutants displayed elevated cyclin E levels and the mutant Ago proteins showed reduced affinity for cyclin E in vitro.
Moreover, spy mutants displayed elevated responses to gibberellin and brassinosteroid, and increased expression of common target genes of DELLAs, BZR1 and PIFs.
Although levels of ACS, VLCAD and PGC-1α were not changed in the TMEM135-deficient animals, we did find that TMEM135::GFP-overexpressing mutants displayed elevated levels of ACS, VLCAD and PGC-1α (Fig. 7).
Both ZO-1 and ZO-2 null mutants displayed elevated levels of apoptosis throughout the embryo (Katsuno et al., 2008; Xu et al., 2008).
Except for the bm2 mutant, all other single bm mutants displayed elevated virulence symptoms after U. maydis infection, with bm3 showing the strongest effect.
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These respiration-deficient mutants display elevated steady-state levels of ROS, probably due to enhanced electron leakage from their defective transport chains, which compromises the viability of chronologically-aged cells.
NS-associated SHP2 mutants display elevated phosphatase activity, while LS-associated SHP2 mutants exhibit reduced catalytic activity.
In these circumstances, the TERT-null mutants display elevated VSG switching driven by gene conversion (Dreesen and Cross, 2006b; Hovel-Miner et al., 2012).
The isi1 mutants display elevated chlorophyll levels and depleted starch, suggesting the inefficient use of carbohydrate resources and perturbation of sugar-responsive gene expression [ 69].
The mutant displayed elevated basal level and induction of the 5′-UTR during acclimation, whereas the 3′-UTR of the transcript was undetectable, indicating that the full-length transcript was absent in sak1.
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