The mutants did not need to produce indole to survive.
In fact, the mutants did not show any signs of oxidative stress or renal failure under catabolic conditions.
Unexpectedly, bdhA and bdhB mutants did not affect solvent production, whereas inactivation of the previously uncharacterised gene CAP0059 resulted in increased acetone, butanol, and ethanol formation.
All the mutants did not change the product pattern, however, the activity was 2-fold increased in S338V, while reduced to half in G256L mutant.
All mutants did not produce quinolones and phenazines in vitro.
dsh-2 mutants did not show any defect (Fig. S4).
Unexpectedly, both GTP- and GDP-locked Rab3a mutants did not promote docking.
Nevertheless, ΔBAR1 mutants did not trigger any symptoms when inoculated onto wheat heads.
On the contrary, well-fed and fasted tph-1 mutants did not show significantly different behaviors.
Overexpression of these GDP-bound inactive mutants did not inhibit constitutive uPAR endocytosis (Fig 8A).
Like earlier mutants, these mutants did not confer cell lethality or temperature-sensitivity (Figure 5A).
