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Molecular methods used for examining the length of telomeres in double homozygous mutants detected much faster telomere shortening than in plants deficient in telomerase gene alone.
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However, in the case of the systematically defined lge deletion strains with broad distributions (boxed and marked with an aster), the density of lge barcode mutants detected was much lower, and many strains were actually detected as whi.
In contrast, PC species that did not contain EPA, PC 20 4/20 4), PC 20 3/20 4), and PC 20 3/20 3), were detected much more strongly in the fat-1 mutants than wild type (Fig. 4c).
C DGAT1 expression levels of different truncation mutants detected by Myc antibody.
Gene redundancy may explain the reduced number of mutants detected.
The mutants detected in each PC seldom overlapped.
I cannot detect much difference.
Plus it's tough to detect, much less mitigate.
Thus, mutant nucleoplasmic RNA is detected at much lower amounts than WT RNA by both Northern and qRT-PCR analysis.
Only four overlapping mutants were detected in the 36 heteroclite mutants (Additional file 7: Figure S5A).
However, in the soluble fraction, the level of the mutant protein detected by mass spectroscopy was much lower than expected.
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