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For visual examination, a minimum of two mutants (depending on the platform) should be imaged from each stage.
Some of these biological processes are specific or common to the two GS mutants depending on the plant developmental stage.
This mechanism could maintain the stomata closed in the esk1-5 mutants depending on nitric oxide signalling.
First, we quantified the levels of remaining AP2 subunits by western blot in each of the mutants (depending on the availability of functional antibodies; Figure 6 figure supplements 1&2).
Determination of transposon insertion sites also enables users to select defined, non-redundant sets of mutants depending on individual needs and facilitates rapid identification of causal genes of the mutant phenotypes.
Given the effect of Rad2p function on cell polarization, we hypothesized that Rad2p overexpression would either enhance or suppress the phenotypes observed in cell polarization and cell cycle checkpoint mutants depending on the role of Rad2p in cell polarization.
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The dynamics of resistant mutants depends on a number of virus replication parameters such as the fitness values, the number of available target cells and mutation/recombination rates.
Thus, the net accumulation of GLR-1 in accretions in lin-10 and rpm-1 mutants depends on GLR-1 endocytosis.
The conditions that dictate the isolation of C. elegans mutants exhibiting enhanced pathogen susceptibility (eps mutants) or enhanced pathogen resistance (epr mutants) depend on the killing mechanism of a given pathogen.
These observations therefore suggest that the restoration of cell viability in E. coli and B. subtilis elongation mutants depends on different mechanisms and that in B. subtilis, this mechanism mainly operates via stimulation of DNA chain elongation.
As expected, mutant cells without pSP1-meiURA4 could not grow on MM plates, whereas mutant cells that were re-transformed with pSP1-meiURA4 grew on MM plates (Fig. 2), indicating that the expression of the ura+ phenotype in the isolated mutants depends on the expression of the ura4+ gene in the pSP1-meiURA4 plasmid.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com