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In contrast to the WT, the insertion mutants demonstrated very low activity against PA83.
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The prkaca-L173A mutant demonstrated very weak growth only at the highest concentration.
As described above, this mutant demonstrated very low enzymatic activity, and the structure showed several features in common with the V266H variant (Supplementary Figures S7A and S7B at http://www.bioscirep.org/bsr/032/bsr0320401add.htm).htm
Further studies on additional SOD1 mutants involving different amino acid substitutions at the same site demonstrated very high variability in aggregation propensities.
The scale demonstrated very good psychometric properties.
Eleven mutants demonstrated X chromosome nondisjunction at 25% or above.
Under SDs, phyB-9 coplantslants flowered at a very similar time to cop1 6 mutants, demonstrating that the early flowering of cop1 6 mutants is not enhanced by loss of function of phyB (Supplementary Figure 3).
In addition, detailed analysis of the force curves revealed very similar spring behaviours for both wild-type and mutants, demonstrating that the sensor mechanical properties are not determined by the CRD domain (Fig. S2).
Mutants demonstrating normal growth in presence of cerulenin were considered as good candidates for further studies.
Indeed, the complete knockout of the dcl1 mutant is embryo-lethal, with partial loss-of-function dcl1 mutants demonstrating less severe developmental defects [68].
xcm9 mutants demonstrate premature termination and bifurcations of shoot apices.
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