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Although acc and beo mutants demonstrate abnormal swimming behavior, C-bends are often observed (Fig. 1C E).
Correspondingly, extracellular peripheral nerve recordings show that que mutants demonstrate abnormal locomotor output to the axial muscles used for swimming.
Given that que mutants demonstrate abnormal behavior and nervous system function consistent with the severe dystonia observed in humans, we sequenced the dbt gene.
Given that our analysis of the free amino acids levels in que mutants showed decreased concentrations of free glutamate and these mutants demonstrate abnormal CNS function and motor behavior, we examined the distribution of glutamate using an antibody.
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The mutation is recessive, and homozygous mutants first demonstrate abnormal rostro-caudal compressions at around 72 hpf (data not shown).
Analysis of zebrafish mutants that demonstrate abnormal locomotive behavior can elucidate the molecular requirements for neural network function and provide new models of human disease.
In Chlamydomonas, CEP135 ortholog bld10p mutants (bld10) demonstrated abnormal interphase microtubules and mitotic spindles, defects during cell division and a significant decrease in the growth rate [ 73]; additionally, CEP135 knockdown in CHO cells showed a decreased growth rate [ 72].
Objective: Complicated pregnancies demonstrate abnormal decidual and placental villous vasculature.
Osteoprotegrin knockout mice demonstrate abnormal remodeling of the otic capsule and progressive hearing loss.
que mutants demonstrate a progressive defect in motor behavior that culminates in abnormal CNS function and accordion behavior.
The pmt4 mutant grew poorly on high salt medium and demonstrated abnormal septum formation and defects in cell separation.
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