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These three mutants could not be exported to extracellular medium.
The mutants could not form biofilms, and only a tenth as many stuck to the beads.
However, uncertain genes disruption may be occurred by random DNA integration during fungal transformation and the phenotype of deleted mutants could not be recovered as expected.
Gliotoxin was found in a recent HTS screen and resistant mutants could not be generated, so the target was not described [9].
Due to the low solubility of β-HCH in water, the kcat and Km values of the mutants could not be calculated.
But the lack of a negative effect of Ot B on the other mutants could not rule out the possibility that Ot B might act on other pathways upstream of DAF-16 [38].
Notably, the BRCA1 mutants could not increase the expression levels of Smad3 protein (Figure 2C).
In contrast, T1 deficient mutants could not inject SopEM45-TEM-1 under all conditions.
As shown in Figure S3B, C, all c-MIR mutants could not down-regulate and ubiquitinate the target molecules.
Hemolysin is known to be required for cellular invasion and cytotoxicity [22]; hemolysin-negative E. tarda mutants could not invade human epithelial cell lines [36].
MqoB and Edd loss-of-function mutants could not utilize ethanol [61] or glucose as sole carbon source, respectively (Figure 6, Tables S4, S5).
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