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An excess of between-dimer mutants could depolymerize preformed wild type polymers, while within-dimer mutants had no activity.
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Lipases and esterases secreted by frankial strains could depolymerize components of plant cell walls, or could modify lipid-based signaling molecules generated by plants in response to stress or pathogen attack [ 31].
For example, immediately following cell division, daughter cells could depolymerize any CtpS filaments inherited to compensate for reduced CtpS concentrations (perhaps from unequal partitioning) faster than translating and folding new proteins.
In both presence and absence of DTT, the MCAK motor could depolymerize microtubules, leading to an increase in free tubulin in the supernatant (S) and a decrease in microtubules in the pellet (P) over time.
Both the 2A and 3A mutants could be rescued by each other and by all of the other mutants tested.
None of the 2B mutants could be complemented by wild-type virus.
WT MDC1 could restore USP15 foci formation, while both deletion mutants could not (Fig. 4k).
We tested whether mutants could predict the time of feeding and show food anticipatory activity (FAA).
As we anticipated, double mutants could not be constructed for the gene pairs SSA_0791/SSA_1494, SSA_0578/SSA_2195, or SSA_0352/SSA_1188, whereas SSA_0169 double mutants were readily obtained (Table 1).
Hence the activity-improved mutants could emerge.
These three mutants could not be exported to extracellular medium.
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