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We next tested whether NKX2-5 mutants could alter off-target gene expression in the inducible ES cell lines described above.
These results suggested that truncated titin mutants could alter assembly and/or stability of the sarcomeric units in human embryonic myocytes.
Since the second phase of decoding involves disruption of the interface between EF-Tu and aa-tRNA, accommodation of the aa-tRNA into the A site, and the subsequent formation of the dipeptide bond, it seemed possible that EF-Tu interface mutants could alter kpep, the rate of dipeptide bond formation.
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Although mutant strumpellin did not destabilise the WASH complex, it remained possible that the mutant protein could alter the complex so that it was unable to bind retromer or localise to endosomes.
The authors proposed that mutant HTT binding could alter DNA structure or sterically block access by other transcription factors and therefore be the initial cause of HD transcriptional dysregulation.
Because eEF1A delivers aa-tRNA to the ribosome and cognate codon:anticodon interactions stimulate its GTPase activity, mutants of this factor could alter translational fidelity in two ways.
Finally, we co-expressed the EYFP-tagged LGMD2H/STM mutants and wild-type TRIM32 in COS-7 cells to determine whether co-expression of TRIM32 could alter the cytoplasmic distribution of the mutants that was reported above (Fig. 3B).
We next examined whether modulating Oxr1 levels could alter the cellular localization of wild-type or ALS-linked Fus and Tdp-43 mutants under oxidative stress.
No letter could alter her fate.
They could alter constitutions as they pleased.
The financial impact could alter quarterly earnings.
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