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We also constructed mutants corresponding to nonclustered glycans or to the full deglycosylation of the V1 or V2 loop.
Seven individual mutants, corresponding to K741R, K741H, K741A, K741E, K741M, K741Q, and K741P, have been engineered and six of the resulting proteins purified to homogeneity.
Arabidopsis mutants corresponding to components not directly involved in HR-type resistance were identified in studies utilizing immunological screens for the levels of viral coat proteins.
The viability of the interaction of αF and αT helixes was confirmed by docking studies and calculation of electrostatic surface potentials for genotype 1 and point mutants corresponding to mutations from different genotypes.
We tested the function of tmRNA mutants corresponding to all possible -1 triplets.
Based on the inferred TRRN and co-expression networks, single gene deletion mutants corresponding to five different transcriptional regulators (CysR, Rgg, GlnR, SpxA, and FabT) were constructed.
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The Arabidopsis ost1 knockout mutant corresponding to the srk2e mutant [18], was kindly provided by Dr. Kazuo Shinozaki.
A shorter chromosomal B-region mutant corresponding to aa 143 210 and covering the B2–B4' repeats, but not the B1 repeat, was constructed using previously described procedures [22].
This line was dropped from analysis; consequently, there is no putative mutant corresponding to tgr7.
A soluble US2 mutant corresponding to aa 15 140 of its luminal domain was sufficient to form a stable complex with the MHC I molecule.
Another choice would be a mutant corresponding to mammalian T37G, which does not undergo the requisite conformation change upon nucleotide binding.
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