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Proteasomes from both rpn1 rpn13Δ mutants contained normal or near-normal levels of the UBL proteins Ubp6 and Rad23.
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We also found that, in the myo1(2xIQ1IQ2) mutant, containing normal Myo1 motor domain but duplicated IQ motifs in the neck, Cam2 localized to eisosomes (red arrows in supplementary material Fig. S4F′-F″) and did not colocalize with Rng3.
Importantly, although prdx-2 (gk169 -mutant animals contained normal levels of fat, the higk169 -mutant in danimals1370) prdx-2 (gk169) double mutants were similar to those observed in daf-2 (e1370) at 15 °contained4), inormaling that PRDX-2 is not required for the increased fat synthesis associated with levelsf IIS.
stau mutants also contain normal numbers of oskMS2/MS2-GFP particles, but the majority of these are tightly localized at the oocyte anterior in a narrower region than the shallow anterior gradient seen in mago, btz, and TmIIgs mutants.
The northern sector contained normal domestic housing.
Pipettes contained normal intracellular solution.
Control wells contained normal medium.
Histologically these tissues pieces contained normal epithelium.
It is noted that all survived mutant pups contain normal nasal septum and palatine when examined at P4, suggesting that the cause of perinatal death is likely due to defects of these two structures.
Also the histopathological examination of the mouse lungs from both WT and the mutant infected mice revealed that most of the lungs of the mutant infected animals contain normal alveolar spaces and airways (Fig. 3D).
The results in Figure 4A showed that each of these mutants contain mostly normal-length ATG31 transcripts; however, low levels of readthrough are apparent in some strains, most obvious in nhp6 (lane 3) and smc3 (lane 12) mutants in the particular blot shown.
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