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Southern blot analysis of GUM1 and GUM2 showed that both mutants contained a single-copy T-DNA integrated into the genome (Figure 3A).
All of the class 1 mutants contained a visible infection thread network in the senescence zone (Figure S2D, S3A and S3B) as well as saprophytic rhizobia invading senescent plant cells (Figure S2C and S2E) similar to what has been described for aging-induced senescence in wild type nodules [17].
The SecA mutants contained a C-terminal His6 tag to facilitate purification without contamination from endogenous, wild-type SecA.
Both single mutants contained a single intron insertion in the chromosome with expected sizes of 4.1 or 4.9 kbp.
Strikingly, all three pH-dependent Fcab mutants contained a mutation next to the histidine located at the last position of the randomized EF-loop region (Table 1).
The purified recombinant angiocidin and its alanine substitution mutants contained a 55 kDa polypeptide that crossreacted with an antiangiocidin monoclonal antibody, as revealed by Western blot analysis.
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The resulted Rubisco mutants contained an average of one to two amino acid substitutions for each of the large and small subunits.
The WT protein as well as all mutants contained an N-terminal Myc-tag and a C-terminal HA-tag and were expressed from a galactose-inducible promoter.
Western blot analysis also revealed that homogenates of cells transfected with both wild-type HGSNAT and polymorphic mutants, but not of untransfected cells or cells transfected with inactive mutants contained an immunoreactive protein with a molecular mass of ∼48 kDa.
Through genetic mapping we found that merovingian mutants contain a mutation in the transcription factor gcm2.
In this study, we reveal that que mutants contain a mutation that disrupts the E2 subunit of the BCKD complex.
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