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Circular dichroism studies of the mutants confirmed that they are structurally similar to the wild-type amelogenin.
Another genome-resequencing results of furfural-tolerant mutants confirmed that the improved furfural tolerance of Z. mobilis mutants could be due to the overexpression of a histidine kinase encoding gene ZMO1162, the disruption of a Sigma-54 modulation protein encoding gene ZMO0038, and the disruption of 1-deoxy-D-xylulose-5-phosphate synthase encoding genes of ZMO1598 and/or ZMO1234.
Southern blot analysis performed on the two trkH mutants and on the 4 auxotroph mutants, confirmed that they (Table 2) contained a single chromosomal transposon insertion (Figure S1).
Experiments investigating the effect of various vir mutants confirmed that the mechanism for transfer uses the same vir machinery as for plant transformation [5], [6].
The immunofluorescence analysis of HA-Grb2 or Myc-GrpL coexpressed with HD-PTP deletion mutants confirmed that the HD is essential for the interaction with the Grb2-family adapters.
Induction of STL1 expression in response to salt stress in hog1 and msn11hot1 mutants confirmed that CaZF functions in a salt stress-dependent manner in yeast and indicates that CaZF also possesses a Hog1p-independent function to activate the expression of some Hog1p-regulated genes.
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In 3T3-TrkA cells, hNGFR100W and hNGFR100E were completely unable to induce the phosphorylation of PLC- γ1 with respect to hNGF (Fig. 1C), unlike other hNGFR100 mutants, confirming that hNGFR100W and hNGFR100E share similar properties.
In the related parasite Leishmania mexicana, knockout mutants confirm that arginase is required for parasite viability, so the inhibition of arginase is a validated strategy in the search for new antiparasitic agents.
Interestingly, the significant reduction in terms of cytotoxicity is not accompanied by a significant reduction in the uptake (~15 20%), binding, catabolism, processing, and presentation of immunodominant peptides to CD4+ or CD8+ T cells from these LLO mutants, confirming that antigenicity of LLO is also independent of its cytotoxic property [ 18].
Confocal laser scanning microscopy (CLSM) revealed that the proportion of biofilm-forming cells decreased substantially in a flo11 mutant, confirming that FLO11 was responsible for biofilm formation.
Addition of OHT only increased the expression of Bcl-2 on cells expressing the wild type E2F1 but not in E2F1E132 and E2F11 284 mutants, confirming that mutants are not able to activate transcription.
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