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In yeast, temperature sensitive eIF5A mutants confer a defect in translation elongation [10].
Compared with the cells expressing wild-type SPT16, most of the spt16 mutants grew robustly in the absence of lysine, similar to what was observed for the spt16 -G132D control, indicating that these mutants confer a strong Spt− phenotype.
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The library was screened for biofilm defects and 31 transposon mutants conferred a reproducible phenotype.
Although most of this new class of spt16 mutants confers an Spt− phenotype similar to many previously characterized spt16 mutants, additional phenotypic studies indicate that there are important functional differences between these mutants.
The phyA-211 phyB-9 double mutant conferred a lower germination percentage rate, both in darkness and after a prolonged white light treatment (Table 1).
Taken together, these results suggested that the FBW7 T205A mutant conferred a growth disadvantage on pancreatic cancer cells both in vitro and in vivo.
Furthermore, using the MM cells with BAP1 deletion, we found that WT BAP1, and even a missense mutant, conferred a higher survival rate after IR compared to the control vector.
Because the Ala mutant confers a 40% higher MnSOD activity than the Val wild-type form, the increased levels of SOD2 result in increased risk for more invasive glioma activity by inducing MMPs.
However, when the experiment was repeated using wild type M. tuberculosis H37Rv and a M. tuberculosis hspR KO mutant [27] the mutation conferred a growth defect relative to the wild type strain during the batch phase of growth whereas there was no additional reduction of fitness observed during chemostat culture at slow growth rate (Figure 4).
None of these mutants conferred any detectable perfringolysin O activity (Table 2), indicating that the mutations had eliminated all measurable VirS function.
We then determined the growth rates of these strains on two types of media to see if the ftsZ mutants conferred any growth disadvantage to these strains.
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