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Mutants characterized in this study reveal intriguing genetic differences between subpopulations of embryonic cilia, and provide an opportunity to study several aspects of cilia structure and function.
Investigation of the clastogen sensitivities of the mutants characterized in this study revealed unexpected variability.
All other spt16 mutants characterized in this study are expressed from plasmids derived from pAO01.
This observation is consistent with the phenotypes of secondary CESA mutants characterized in other grass species [ 22, 23, 25].
Compared with the mutants characterized in our study, strains used for QTL mapping typically have more segregating sites and more causative loci.
Of the 1,604 unique BR mutants characterized in the directed evolution search, eleven qualify as bBR membranes, and one mutant (D85E/D96Q) exhibits both high stability and the ability to fully switch between the bR B and Q photostates.
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In addition to the apoc2 mutant characterized in Figs 1- 4, we generated a second zebrafish line with a different apoc2 mutation, which introduced a stop codon in the target site, causing loss of the Lpl-binding domain of Apoc2.
Although Ciona mutants characterized by an impairment in tail extension have been previously described, such mutations are attributable to specific defects in notochord development [23], [24].
Of the mutants characterized, M1 was the most consistent in response across NB and PC12x cells and nuclear extracts.
The mutants were characterized in vivo using the model organism S. cerevisiae as it affords a genetic background that can be readily manipulated.
Ten of these mutants were characterized in some detail.
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