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The mutants carry a T-DNA insert in the second intron of At5g40390.
These all 17 mutants carry a Y chromosome because at least one of Y-linked marker is present.
Celsr1 Scy mutants carry a single-base substitution within Celsr1, c.3337T>A, causing the amino acid change N1110K (Curtin et al., 2003).
Taken together, trg mutants carry a recessive lethal mutation that causes severe muscle weakness and a uniform reduction in birefringence, indicating deficiencies in the contractile apparatus of the muscle.
These mutants carry a single autosomal P[GT1] transposon insertion in the respective miRNA genes in a w ; Canton-S (w [CS]) wild-type genetic background (Yamamoto et al. 2008).
This proportion indicates that ∼96% of mutants carry a unique transposon insertion in their genome and there is no significant redundancy of a clone arising from identical parent clones in the same tri-parental mating experiment.
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Extended Data Fig. 2 The GCR is triggered in capn3a genetic mutants carrying a functional PTC.
None of the mutants carried a mutation in soxR.
One of the mutants carries a defect in Sch9, which resembles two genes in the C. elegans daf-2 pathway.
Mutants carrying a deletion in either CWC1 or CWC2 are blind to the inhibitory effect of light on mating and are sensitive to ultraviolet light.
Notably, yeast mutants carrying a γCOP allele with a similar C-terminal deletion (579 aa) are not viable; expression of this truncated protein may also exert a dominant negative activity.
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