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None of the mutants carried a mutation in soxR.
Specifically, only 18% of the screened EZ-Tn5 C. perfringens mutants carried a transposon insertion into a rRNA gene (rRNA genes represent about 1.5% of total genes in C. perfringens).
In the absence of antimicrobial pressure, 6 out of 11 mutants carried a selective advantage over the antimicrobial-sensitive parent strain, indicating that FQ resistance in S. Typhi is not typically associated with fitness costs.
As shown in Table 2, mutational frequency (MF) in repair and replication proficient strain was 6.2% and 8.4% without and with SOS, respectively, and equal fraction of mutants carried A → T and A → G in each case.
We applied the WGS/SNP mapping strategy to seven legacy mutants and found that all seven of the mutants carried a single-point mutation resulting in a nonsynonymous amino acid change that caused the mutant phenotype, six of which were temperature-sensitive.
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A few of the haploid deletion mutants carried an additional wild-type copy of the gene deleted, likely due to duplication of all or part of the chromosome.
One of the mutants carries a defect in Sch9, which resembles two genes in the C. elegans daf-2 pathway.
Mutants carrying a deletion in either CWC1 or CWC2 are blind to the inhibitory effect of light on mating and are sensitive to ultraviolet light.
Notably, yeast mutants carrying a γCOP allele with a similar C-terminal deletion (579 aa) are not viable; expression of this truncated protein may also exert a dominant negative activity.
In Drosophila, mutants carrying a deletion of the Mnn1 gene are hypersensitive to several mutagens or gamma irradiation and display profound defects in the stress response [5], [16], [21].
The mutants carry a T-DNA insert in the second intron of At5g40390.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com