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From the functional point of view, gas1p is essential for cell integrity and viability during vegetative growth, since gas1Δ mutants can only grow in osmotically supported media, while gas2p and gas5p play a minor role in cell wall construction.

Such mutants can only be maintained as heterozygotes in the laboratory [ 16, 33].

Most of the double mutants can only grow at 25° but not 37° as predicted.

In fact, these mutants can only be bred for one generation owing to their reduced fitness levels.

As all these reactions in those mutants can only be accomplished if the heme bL– bL electron transfer takes place, it is clear that the kinetic traces proved that these two hemes exchange electrons on a catalytically-relevant timescale.

As we do not know how the WD repeat units of the HNWD proteins assemble, the reasons for the loss of activity in the mutants can only be speculated, but it is reasonable to suspect that loss of the genetic incompatibility in the mutants results from alterations of the physical interaction between the WD repeat domain of HET-R or HET-E proteins with their antagonist partner HET-V or HET-C.

Similar(54)

Like a complemented gD null mutant, a complemented gB null mutant can only infect once as its progeny do not contain gB and are noninfectious.

Therefore, the significantly lowered CI after 48 h for the double ΔfadD2D1 mutant can only be due to a reduced ability to degrade PC as a nutrient source for replication in the mouse lung.

This is because competition of the beneficial mutant can only occur at the edge of a colony, and as the colony grows, a smaller proportion of the mutant population will be competing with the ancestral genotype (Habets et al. 2007).

Pro sensitivity and stress induced Pro accumulation differed between the Col-8 and L er wildtypes, confirming that a useful double mutant can only be produced after extensive backcrossing.

For example, starting from one mutant cell, a mutant colony can only spread as a solid, connected spot.

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