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We identified γCOPP1 mutants by screening a collection of lethal P-element insertions for tracheal tube size defects [21] [25].
For the COMT1 gene, Xin et al. [ 11] screened 768 EMS mutagenized lines of sorghum by TILLING and found two mutants by screening 624.4 kb.
We identified potential K+ homeostasis mutants by screening the yeast homozygous diploid deletion collection, which represents about two-thirds of the genome and includes all nonessential genes, for sensitivity to hygromycin B. Although hygromycin B is a translation inhibitor (Brodersen et al., 2000), ribosomal protein genes are for the most part essential and so not present in this collection.
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In addition, they identify an ush1c zebrafish mutant by screening DNA from individual mutagenized fish to find a lesion in the ush1c gene.
In both E. coli and S. enterica, it was possible to identify a correct mutant by screening fewer than a dozen colonies when the selection cassette contained a double terminator element, whereas it was necessary to screen forty colonies to find a correct mutant when the selection cassette contained a single terminator element (Table 3).
The collection has an extremely high mutation rate that enables the detection of large numbers of independant mutant families by screening a comparatively low number of M2 families.
The P. s ojae susceptible (pss) 1 mutant was identified by screening a mutant population created in the Arabidopsis pen1-1 muthat that lacks penetration resistance against the non adapted barley biotrophic fungal pathogen, Blumeria graminis f. sp. hordei.
hip3-1 mutant was isolated by screening for suppressor mutants using a reporter plasmid.
Using a standard procedure for transposon insertion mutagenesis of SCHU S4 [34], a total of 743 mutants were generated by screening on plates containing kanamycin.
Thirty-one mutants were isolated by screening 1,800 transformants generated by random insertional mutagenesis (1.7%).
These mutants were identified by screening progeny from self-fertilized "wild-caught" Ciona.
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