Sentence examples for mutants by replacing from inspiring English sources
The entire coding sequences of gas2+ and gas5+ were deleted to create the null mutants by replacing them by the KanMX4 cassette using the same approach.
Similarly, we have created additional Magmas Ts mutants by replacing conserved residues at the base of the helix III region.
We generated single amino acid mutants by replacing each of positions Φ3, Φ4, and Φ5 in hRio2NES with other hydrophobic residues, and tested binding of the mutants to CRM1.
We constructed single knock-out mutants by replacing these three genes with a gentamycin resistance cassette and compared these strains to the wild-type and the QS deficient strain.> All deletion strains were viable and as predicted by our hypothesis their phenotypes were comparable to those of the QS defective mutant.
They selected 18 lncRNA genes in the mouse genome that had been stringently assessed for lack of protein-coding capacity and that did not overlap with known protein-coding genes or other known gene annotations hence the name long intergenic noncoding RNAs (lincRNAs)—and generated knockout mouse mutants by replacing the lncRNA gene with a lacZ reporter cassette.
We made a GABARAPL1 LDS mutant by replacing Y49 and L50 with alanines.
To test this idea, we created a catalytically inactive DUSP6 mutant by replacing the critical cysteine-293 with serine (C293S mutant; Levinthal and Defranco, 2005).
Attempts to construct a deletion mutant by replacing the A. fumigatus nmt gene with a pyrG selection marker and phenotypic rescue by supplementing the culture medium with myristic acid failed.
To investigate whether HEL67 protein has a role in asrRNA fragmentation, we generated a L. infantum null HEL67 mutant by replacing the HEL67 gene by two different selection marker genes.
Firstly we generated a new null sltB mutant by replacing the sltB coding sequence with the riboB gene from A. fumigatus (riboB Af) and using strain MAD1427 as recipient.
To address the importance of DKS sequence in the loop connecting helix II and helix III (Fig. 1B), we have created a triple mutant by replacing it with the canonical J-protein conserved histidine, proline, aspartic acid (HPD) sequence.
