Sentence examples for mutants by introducing from inspiring English sources
We generated extracellular signal-regulated kinase 1/2 (ERK1/2) mutants by introducing a single amino-acid substitution in subdomain V of the catalytic domain and then examined the susceptibility of these mutants to PP1 derivatives originally designed as Src inhibitors.
Similarly, we depressed endocytosis in lin-10 pmk-3 double mutants by introducing the Pglr-1::rab-5(GDP) transgene.
Therefore, we generated two DNA binding deficient TWIST1 mutants by introducing the S144R/K145E and R118C mutations.
We tested the function of our binding mutants by introducing the proteins into an eco1-1 strain from which WPL1 had been deleted.
Complementation of the mutants by introducing parS-parR in trans on a medium-copy-number vector reduced phenazine production below wild type levels, confirming its negative regulatory role in phenazine biosynthesis.
To test this hypothesis, we engineered complementary enzyme and substrate mutants by introducing hydrophobic residues of different side chain volumes to position 146 of GlpG (F146A and F146I) and by testing their activity against all 20 possible mutations in the P4 position of TatA substrate.
Expression vectors encoding the C6A/C111S/G93A (andG93A) and C6A/C111S/G85R (AS/G85R) mutants were derived from the expression vector for the AS (C6A/C111S) mutant by introducing appropriate point mutations with the QuikChange Mutagenesis Kit (Stratagene).
We also mimicked the V266H mutant by introducing steric clashes elsewhere in the allosteric network, generating several mutants with reduced activity.
To examine the cooperative role of the OSR family members in organ growth, we generated an osr2 argos-1 ARLi triple mutant and an argos-1 osr1 ARLi OSR2i quadruple mutant by introducing a pro35S OSR2 RNAi construct into argos-1 osr1 ARLi plants.
Consistent with previous findings, complementing the oryR − mutant by introducing the oryR gene in the high-copy plasmid pBBROryR significantly decreased swimming as compared to WT bacteria, a phenomenon which is likely explained by the overexpression of oryR in a multicopy plasmid and the self-negative regulation of oryR [ 43].
In future, a more prolonged rescue might be obtained in vivo by upregulating Gdnf/Ret signalling in Fras1 mutant mice by introducing a heterozygous Sprouty1 mutation, as used to rescue Itga8 mutants (22).
