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No detectable differences were found in vegetative growth or floral organ development between Nipponbare and loss-of-function mutants, but the pollen grains of all loss-of-function mutants were aborted (Fig. 4d-g and i-l).
If we increase the death rate, the total number of cell divisions to reach size N will increase, and so will the number of mutants (but the average size of a clone size will remain the same).
At later stages, when myoblast fusion has initiated, lms expression does appear in the precursors of the LT muscles in msh mutants, but the expression is still reduced or absent in many of the segments (Fig. 3E).
We attempted to examine this possibility through determination of genome-wide occupancy of Rsc1 and Rsc2 in rco1Δ mutants, but the data was inconclusive due to inconsistent results between biological replicates (data not shown).
Here, we have explored the efficacy of PAC to identify exon-skipping mutants, but the strategy may also be used to deduce the primary structure of gene transcripts [13], [22].
Plasma cholesterol levels were slightly lower in mutants, but the difference did not achieve statistical significance (Fig. 1B) (132±17 mg/dl in wild type vs. 115±24 in mutants, n = 10, P = 0.101).
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K103N + L100I is the most drug-resistant of the double mutants but is the least common clinically.
Alternatively, CaZF can partially complement salt sensitivity of the can mutants but requires the CAN pathway for its full function.
A second list was generated using the same mutants but using the pSDC values instead.
All control groups as well as the α6-Cacna1a mutants, but not the Pcd, L7-Pp2b and L7-∆γ2 mutants, improved their efficiency with training (for p values, see Table 1).
Indeed, the SbIII-, MF- and AMB-resistant mutants, but not the PARO-resistant mutant, became more easily cross-resistant to the apoptosis-inducing drugs SbIII, MF and AMB.
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