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Viral quasispecies are not a simple collection of diverse mutants but a group of interactive variants capable of adapting to new environments [32].
Overexpression of these mutant proteins occurred on addition of anhydro-tetracycline (Figure 3A, L245SR25D data shown are representative of results for all three mutants), but a concomitant increase in transcription of clpP1P2 was not observed (Figures 3B and C).
Pep4-GFP localized to the class E compartment in ESCRT mutants, but a large fraction of Pep4-GFP was also delivered into the lumen of the vacuole.
We observed normal MI dynamics in rec12Δ mutants, but a reduced efficiency of MII, similar to previous reports (Fig. 2A) (Davis and Smith, 2003).
The fact that all these genes were identified validates our screening approach and emphasizes that it is not one gene or pathway responsible for life extension in Mit mutants, but a network of genes that must be specifically modulated.
We selected Glc7 for our analyses since a framework of genetic and protein interactions exists from previous studies of conditional glc7 mutants, but a comprehensive large-scale genetic analysis had not yet been performed.
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Vegetative wildtype cells perform macropinocytosis in a qualitatively similar way as axenic mutants but to a lesser extent, and accordingly they can grow axenically when the standard medium is supplemented with bovine serum.
We observed about a 10-fold increase in binding of Stn1 and a 5-fold increase for Ten1 to telomeres in nmd2Δ mutants but only a 2-fold increase in the levels of Cdc13.
This could be mentioned.Figure 4: Eater is not a cellular deficient mutant but a phagocytic mutant.
At the Dio1 gene for example, two HNF4A binding sites were lost in the mutant, but a third was unchanged.
Unexpectedly, we detected a fitness defect for the pba4 / poc4 heterozygous deletion mutant but a fitness advantage for the pba3 / irc25 heterozygous deletion mutant.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com