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WT and brm mutants both in the Col and the Col FRI background were vernalized for 40 days then transferred either to long days or to short days (only Col plants) and flowering time was measured (Figure 4A).
A mild early flowering time phenotype was observed in the lif2-1 to lif2-3 mutants both in short-day (SD) and long-day (LD) conditions (Fig. 3D) (Table 1).
These tools are based on the possibility of generating collections of single gene mutants –both in unicellular [8], [9], [10], [11], [12] and multicellular [13], [14], [15] model organisms, and also in mammalian systems [16], [17] – that are then queried against a second large set of target gene mutations (Figure 1A and Box 1).
The generation and examination of these mutants both in vitro and in vivo can provide high-resolution analysis of biological processes and cell phenotypes and provide new insight into the evolution and molecular function of this important protein family.
Furthermore, when we compared the flowering times between atjmj4-1 single and atjmj4-1 flc-3 double mutants both in LD and SD, the double mutants flowered earlier than the single mutants in both photoperiodic conditions (Figures 3A and 3B).
In agreement, immunostaining showed reduced NRP1 protein levels in the mutants, both in axons and vessels (Fig. 2B,C).
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MtN19 activation was strongly reduced in the efd-1 mutant both in roots (this study) and in nodules [26], making it a possible target gene of EFD.
DMM carried out the production and purification of ChU-B mutant both in P. pastoris and S. cerevisiae.
Consequently, it is absolutely necessary to show the phenotype of the akin10 mutant both in the light and dark that we can compare that to that of the bzip63 mutant.
The selective inhibition of TrkA signaling by hNGFR100 mutants occurs both in the absence or in the presence of p75NTR.
In the present study, the pharmacokinetics and immunogenicity of one or more 5mg/kg subcutaneous (SC) injections in naïve macaques of the HIV bnAb PGT121 and its PGT121-YTE mutant, both produced in plants, have been compared towards prolonging efficacy.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com