The Akt pathway is most commonly associated with cell survival, so we decided to investigate whether apoptosis may be increased in the OCRL1 mutant beyond the naturally occurring level seen in embryogenesis.
Although the phenotypic characterization of the SBEII mutants is beyond the scope of this work, we have successfully confirmed each WKS and SBEII mutation in its corresponding M3 seed stock.
Such a function would contribute another dimension to the cerebellar pathology as well as provide an explanation for the persistent cerebellar effects of the PEX13 brain mutants beyond P30.
Our approach extends the applicability of NGS-based mutant analyses beyond the domain of available reference genomes.
Interestingly, the mog genes and ife-3 share mutant phenotypes beyond the mog.
While complete mapping of the nature FUDR resistance in this mutant is beyond the scope of this study, these findings highlight the power of chemical mutagenesis and phenotypic screening to identify both expected and unexpected biological targets.
Importantly, some impairment of cerebellum foliation persisted in mutants beyond P20, until at least P30 (declival, absent in 1/3 animals; intercrural, absent 0/3; uvular, absent 0/3) (Fig. 5I,J; supplementary material Table S2).
Within the OFT, NKO mutant NCCs migrated beyond the conotruncal junction and invaded entire layers of the OFT.
However, we hope the editors agree that the double mutant experiments go beyond the scope of this already rather extensive manuscript.
