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This phenotype might be explained by the reduced motility of macrophages in cxcr3.2 mutants, because it has been shown that early granulomas in zebrafish larvae expand by spreading of the infection to newly recruited macrophages (Davis and Ramakrishnan, 2009).
Based on the initial assessment of antiviral mutagens, ribavirin was selected for further study to evaluate the possible emergence of ribavirin-resistant mutants because it showed the greatest effect on PRRSV replication without causing significant cytotoxicity even at the highest concentration (1.5 mM).
Strain E was unique among the mutants because it had only the three bands common to A through F (none at the same location as the three bands in the wild-type strain); all the other mutants had a total of four main bands except B2, which had two main bands (also seen in wild type).
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The sug-h mutant has a mild sugary phenotype, which preferentially accumulates desirable sugar compositions of non-starch polysaccharide rather than starch, and is more commercially viable than the sug-1 mutant because it does not display excessive wrinkling, which interferes with milling (Koh and Heu 1994; Yoon et al. 2009).
In the end, she concluded that the episode was "part of the X-Files, moreso than would an alien or a mutant" because it focused on the idea that "pain makes you, more pain makes you better, and living within that pain keeps you alive".
We didn't evaluate the ME of the triple mutant because it was created without a six-histidine tag and therefore could not purify it from the crude bacterial lysate to measure the ME based on protein concentration assay.
We tested this cyclin D1 mutant because it is resistant to polyubiquitination, and also prevents the nuclear export of cyclin D1 during S phase resulting in constitutive nuclear localization [9].
Thus, the defects may be greater in the rom2 ∆ mutant because it impacts multiple pathways, whereas wsc1 ∆ only impacts a subset of pathways (Popolo and Vai 1999).
The rational is that any atrad21.3 induced DNA dsb damage phenotype (that may go unnoticed in the atrad21.3 single mutant because it is masked by the function played by AtRAD21.1) will be more easily detected in the double mutant.
We focused subsequent analyses on the cpi1-1 mutant, because it displays the strongest alteration in sterol composition of known sterol biosynthesis mutants (Men et al, 2008) and revealed KNOLLE mis-localization at the lateral PM in 43% of late cytokinetic cells.
To verify that the transcription start site of our readthrough transcript initiates in the region of the annotated SUT467 transcriptional start site (TSS) and to map the TSS of the observed intermediate transcript, we performed 5′-RACE analysis on RNA isolated from the brf1 -II.6 mutant, because it contains all three transcripts as detected by Northern blotting.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com