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In the forward screen, we selected five highly tolerant and four highly intolerant mutants based on the response of seedlings to 300 ppm Fe.
The tagged ST556 mutants were assembled in 96 sets of 55 mutants based on the sequence identity of the tags.
A series of previously generated mutants, based on the GFP-UNG2 construct [50], [51], were transfected into human 143b cells.
To investigate this further, we designed three hOGT truncation mutants based on the structural data.
We created three groups of XMRV RNase H mutants based on the structural information on XMRV RNase H.
This discrimination of mutants based on the structural landscape mirrors phenotypic variation seen by GFP-tagged aggregates (Couthouis et al., 2009) (independent of predictive accuracy).
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Yet we cannot exclude that less efficient salvage pathways mediated by Kitb and Csf1rb compensate for the loss of Kita and Csf1ra function in the mutants based on these findings.
Serine 156 or Asparagine 185 was replaced with alanine or aspartic acid, respectively, by PCR-based site-directed mutagenesis to produce the S156A or N185D AQP5 mutant based on the AQP5 pconstructonstruct as previously described [13].
We report here a Ser127Ala mutant based on the enzyme glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes.
This result favors an alternative explanation to the low levels of P110 in the T192 mutant based on the genetic structure of the transposon insertion in this strain.
However, defective epithelial differentiation was detected in the mutant based on the expression of keratin markers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com