Mutagenesis was followed by the screening of mutants based on cerulenin as an attempt to obtain mutants displaying increased lipid productivity.
While it would be preferable to use wild strains for wine fermentation studies, mutants based on these strains are not yet available.
We investigated the efficacy of CNS transduction using a variety of tyrosine and threonine capsid mutants based on AAV2, AAV5, and AAV8 capsids, as well as AAV2 mutants incapable of binding heparan sulfate (HS).
Spatial light modulation (SLM) has been theoretically analysed in pharaonis phoborhodopsin (ppR) and its mutants based on nonlinear intensity induced excited-state absorption, to achieve large percentage modulation at low power.
While numerous computational methods have been developed that use genome-scale models to propose mutants for the purpose of metabolic engineering, they generally compare mutants based on a single criteria (e.g., production rate at a mutant׳s maximum growth rate).
In order to enable structure-based design of IKK-2 inhibitors, we carried out a rational generation of IKK-2 mutants based on induced-fit docking of a selective IKK-2 inhibitor, PHA-408, into the homology model of IKK-2.
The classification of mutants based on secondary structure (helix, strand, turn and coil) and solvent accessibility (buried, partially buried, partially exposed and exposed) distinguished the stabilizing/destabilizing mutants at an average accuracy of 81% and 80%, respectively for ΔΔG and ΔΔGH2O.
The tagged ST556 mutants were assembled in 96 sets of 55 mutants based on the sequence identity of the tags.
A series of previously generated mutants, based on the GFP-UNG2 construct [50], [51], were transfected into human 143b cells.
