Ai Feedback
Exact(6)
We also assayed phosphorylation states of the thylakoid proteins in the stn7 and stn8 mutants at these time points representative for night and day (Figure 3B).
However, we noticed that the general phenotypic features observed with rpl35 knockdown were common among the RP gene mutants at these later stages of embryonic development (2 4 dpf), when morphogenesis of primary organs systems is complete.
To evaluate this hypothesis, structures of reciprocal single-site mutants at these 15 positions around the active site of BSP165 and TF MAN in complex with mannotriose were constructed and pKa calculations were carried out (Table 4).
To explore the influence of the conserved charged residues on phospholipid transfer by PRELI-like domains, we decided to test mutants at these positions for function.
This was also the case for Phe37, Ala68, Gly292, Asn293, Phe373, and Tyr374 (some or all of the mutants at these positions had lower percent conversion values, but all those retaining activity showed essentially unchanged S stereoselectivity for 1– 3).
To test if the targeted residues affect formation of the E S complex, we determined the change in salt dependence for SRL cleavage by mutants at these positions [Δ n = nWt − nMut (Table 1 and Figure 1)].
Similar(54)
Interestingly, specific binding was retained for both of these mutants at the AM2 receptor, in line with the cAMP data.
We go on to investigate the in vitro biochemical and biophysical properties of these mutants at the molecular level.
However, despite the normal density of cilia in mutant retinas, cilia length appeared decreased in mutants at the periphery of these whole-mount eyes, where the cilia can be viewed tangentially (Fig. 4E and F).
These effects correspond with the decrease in binding affinity of AM to these mutants at the AM1 receptor ECD, as determined using surface plasmon resonance (Kusano et al., 2012).
Dominant-negative mutation of JNK (DN-JNK) is a mutant at these sites (on Thr and Tyr), which effectively suppresses paclitaxel-induced cell apoptosis in cancer cells [ 10].
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com