Sentence examples for mutants at position from inspiring English sources

Replacement of Tyr224 by phenylalanine results in inactive enzyme, whereas mutants at position Arg373 retain catalytic activity close to wild-type level.

Further, pH-activity profiles of mutants at position 91 and 226 were analyzed.

In addition, the mutants at position 114 have been shown not to regenerate with 11-cis-retinal [33], [39].

Four mutants at position 139 were tested.

Moreover, the activities of the mutants at position 142 for various substrates indicated that the position was closely related to substrate specificity.

The selectivity could further be enhanced by direct screening of Hxt11 mutants at position N366 for glucose-insensitive transport of xylose.

Mutants at positions −133 and −418 (TT mutated to TG) were generated by using the QuickChange Site-Directed Mutagenesis kit (Stratagene) and confirmed by sequencing.

In order to investigate the role of the FeS cluster-chelating cysteine residues in TFEβ we produced cysteine to serine mutants at positions C92, C95, C101 and C112.

Having established this, we proceeded to incubate double mutant GFP S147C, T230C, 233Δ) 8 with 2,5-dibromohexanediamide (4, 50 eq., 2 h, 37 °C) to see if the results observed on the single mutants at positions 147 and 230 would be translated.

The data from MD simulations presented here for the allosteric mutants at positions 55 and 128 show a change in mobility of active site loop 1, suggesting that the loop dynamics are critical for maximal enzyme activity.

For site-directed mutagenesis, the following primers were utilized: Sebox 5 (new_mutant at position 2 generating the second G): 5′ GATGATGAACTC TTCACgTCCCACACG 3′; Sebox 6 (new mutant at position 3 generating the first E): 5′ GATGATGAACTCTTtACCTCCCACACG 3′; Sebox 7 (new_mutant at position 4 generating the second E): 5′ GATGATGAACTtTTCACCTCCCACACG 3′.