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We were unable to detect differences in either the level or location of p-Smad staining in either the mesenchyme or ureteric buds of Lrp4 mutants at either E10.5 or 11.5 (data not shown).
Earlier work had demonstrated that BidBH3 M97 contacts Bax I66, and alanine mutants at either or both of these positions reduced the capacity of the peptide to release the core domain from the latch domain.
That the rate of virion assembly does not differ between the ancestor and the mutants at either temperature suggests that capsid assembly is not the rate-limiting step once virion assembly begins.
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DC3000 avrRpt2 elicited no statistically significant induction of PR-1 by the double mutant at either time point (Student's t test, P > 0.05).
DC3000empty vector did not elicit significant PR-1 induction on the double mutant at either time point (Student's t test, P > 0.05).
These results demonstrate that wild-type WRN or the WRN exonuclease dead proteins are similarly able to restore the top3 growth phenotype in the sgs1 top3 background, whereas the WRN mutant proteins that have defective ATPase/helicase activity did not affect the growth of the sgs1 top3 mutant at either low or high levels of gal-induced protein expression.
Most wasp eggs dissected from w1118 controls and homozygous mysnj42 or Rac1J11 mutants, raised at either 22°C or 29°C, were fully encapsulated by plasmatocytes (data not shown).
To explore this possibility, we measured the lifespan of N2 and mir-83 (n4638 ); mir-34 (gk437 ) mutants raised at either 20° continuously or with temperature oscillations during the time-sensitive period.
As seen in Figure 4 there was no significant difference in β-galactosidase activity between the wild-type and sdiA mutant strains at either 30°C or 37°C.
Somewhat surprisingly, only 2 genes met our criteria of being differentially expressed (fold-change ≥1.5-fold, P-value≤0.05) between MGAS2221 and isogenic mutant 2221ΔPEL at either time-point (Figure 7B and data not shown).
Similarly, we find that mutant alleles at either locus can invade these stable equilibria.
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