Sentence examples for mutants as previously from inspiring English sources

These plasmid constructs (pSF-VasH, pSF-MsbB, pSF-CirA, pSF-Uge, respectively) were used to obtain vasH, msbB, cirA, uge deficient mutants from A. hydrophila strain AH-3 by a single recombination event leading to the generation of two incomplete copies of wild type genes in the chromosome of these mutants, as previously described [41].

The P. falciparum multidrug resistant strain Dd2EF1 was cultured in triplicate in the presence of increasing concentrations of GNF-Pf4492 to generate resistant mutants as previously described.

However, functional redundancy can only be assessed in combined mutants, as previously demonstrated for the lethality and defects in neuromuscular or cortical development observed in double and triple knockouts [ 20, 21, 46].

We performed temperature stability assays to examine whether the introduced amino acid substitutions caused detrimental effects on the tertiary structure and to stability of chagasin mutants, as previously reported to occur with cystatin C, another CP inhibitor [15].

The Fluor-de-Lys tetrapeptide assay substrate Ac-Arg-His-Lys Ac -Lys Ac -aminomethylcoumarin (BML-KI178-0005, Enzo Life Sciences) wAc-Arg-His-Lys Ac -Lys Ac -aminomethylcoumarin HDAc-Arg-His-Lys Ac -Lys Ac -aminomethylcoumarin

Human HA-tagged MEK1 and MEK2 cDNA constructs were used as templates for in vitro mutagenesis to generate the constitutively activated MEK1 S218D/S222D) (MEK1DD) and MEK2(S222D/S226D) (mutants mutasts as previously reported [ 33].

Plasmids pSF-VasH, pSF-MsbB, pSF-CirA, and pSF-Uge, were independently isolated, transformed into E. coli SM10 (λpir) [41], and transferred by conjugation from E. coli SM10 to the AH-3 rifampin-resistant (Rifr) mutant as previously described [17].

This phenomic analysis confirmed and strengthened previous data obtained by Buroni et al. [18] on mutant D4, showing that RND-4 is involved in the extrusion of a wide variety of compounds toxic for cell metabolism, in agreement with antimicrobial susceptibilities of the mutant as previously determined [18].

T370A and D224N are CDK11p58 kinase dead mutants whereas T370D is a kinase-activated mutant as previously reported [ 12, 18].

The templates used for structural model building were assigned according to the corresponding reference models for each mutant as previously mentioned.

The RNAi rescue experiment was performed by transfecting the ARMS RNAi-2/B16-F10 stARMS RNAi-2/B16-F10 ARMstableA3.1clone 4849T → C expression mutant as previously described (Liao et al, 2007).