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However, given Mary's fascination with the genetic variants and anomalies that mutagenesis can produce, it seems inevitable now that she would establish an interest in the mouse mutants arising from these radiation studies.
Sulfur K-edge XAS studies of wild-type CpRd and the four Ser mutants revealed an increase in the pre-edge energy of the Cys for all four mutants compared to the wild type, indicating higher d orbital energy for the mutants, arising from the more electronegative olate serine ligand, which will lower the reduction potential as observed experimentally.
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These findings support the proposition that habituation defects in rpm-1 mutants arise from defects in neuronal development.
We conclude that craniorachischisis in all three PCP mutants arises from a common embryonic defect, affecting the neural plate midline.
Perhaps this change in the pattern of chromosome segregation in pat1 mutants arises from premature microtubule kinetochore interactions due to a defect in outer kinetochore disassembly.
Successful conjugation-based plasmid transfer to C. pasteurianum has also been documented [ 20], yet no protocol has been described, nor have any genetic mutants arisen from this work.
Additionally, our results show that combinations of these mutations exhibit higher Darwinian fitness than would be expected under a scenario of independent fitness effects, showing that higher fitness in gyrA and parC mutants arises from synergistic epistasis.
To show whether behavioral phenotypes of robo mutants arise from a defective output of the central pattern generators (CPGs) for exploration, independent of sensory input, I performed calcium imaging experiments on the isolated nervous system.
The estimated frequency of the three mutant types suggest that even the commonest double mutant should occur at a discontinuous rate of ∼10−12/cell/division, yet 100 colonies containing such mutants arise from of 10 plated cells, which grew to 10 cells during day 1.
To determine whether adult lifespan extension in oga-1 mutants arises from excessive O-GlcNAc-modifications, we analyzed the lifespan of the oga-1; ogt-1 double mutant which has a markedly reduced level of O-GlcNAc-modified proteins similar to the ogt-1 mutant (data not shown).
For instance, allelism studies established that many of the sorghum bmr mutants arose from deleterious mutations in caffeic acid O-methyl transferase (COMT) [ 22, 23], although evidence of sorghum bmr arising from cinnamyl alcohol dehydrogenase (CAD) were also discovered [ 24- 26].
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