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For a long time, the identification of gene functions has been based on classical forward genetic screens where mutants are randomly generated, e.g. by chemical or transposon mediated mutagenesis.
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Another subset of 25 double mutants was randomly chosen from combinations of this 20 single mutants.
To assess AA content in leaf tissue, seeds of wild type and mutants were randomly sown on MetroMix 360 soil (BFG supplies Co., Burton, OH) in the same flat under the growth conditions described above.
Twelve individual colonies for each mutant were randomly picked and expanded.
In our previous study, the lactose-fermenting ccpA mutant was randomly mutagenized using pGhost9::IS S1.
To analyse the role of each of the four loops in the BtR175-binding mechanism of Cry1Aa, more than four phage-displayed Cry1Aa loop-mutants were randomly selected from each library (from each replacement site) and produced in E. coli as inclusions using the fusion protein connected to GST.
However, in the Vangl or other PCP mouse mutants, cilia are randomly distributed around the center of the node cells, although Nodal cilia are mostly posteriorly localized in wild type embryos [ 51, 54].
Mutants are generated randomly like the individuals of the initial population.
Unlike transgenic mouse models in which multiple copies of a mutant gene of interest are randomly inserted into the genome, the knock-in technology uses homologous recombination to exchange one of the two normal copies of the mouse Acvr1 gene with a copy of the mutant gene in its endogenous chromosomal location, thereby preserving the precise genetic and molecular regulation of the target gene.
To calculate the relative fitness of mutants in pure culture, mutant populations were randomly paired with pure wild-type cultures from the same experimental block and the same formula used.
At the end of the selection, chtB was one of the mutants that were randomly chosen to be tested for cheating properties.
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