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Full details on fatty acid profiles of these mutants are provided in Table 1.
All experimental details of expression profiling the deletion mutants are provided in Additional file 3.
Details for the generation of the H3-L61X mutares are provided below.
Primers used to identify homozygous T-DNA mutants are provided (Additional file 1: Table S6).
The sequences of piRNAs and their corresponding mutants are provided in Supplementary information, Figures S5B and S9A.
The experimental procedures used for the construction of the P1A, R70A, R73A, and E114Q Cg10062 mutants are provided in the Supporting Information.
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In addition to predictive power, MD simulations of multiple RT mutants are providing fundamental insight into the dynamics of the allosteric NNRTI binding site which is useful for the design of future inhibitors.
The list of FST genes with related gene-indexed mutants is provided in Additional file 1: Table S1.
GFP-tagged full-length BRCA1 and BRCA1 deletion mutants were provided by Dr. N. Chiba [68].
A list of primers used to generate and confirm the allelic replacement mutants is provided (Table 3).
The cgt-1 tm1027) cgt-1 tm1027504) mutands cgt-3 tm504 cgt-3 tm504tani Lab (National BioResource Project, Tokyo, Japan).
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