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Mutants are produced through the use of wildcards as well as by using naming parts of original pointcut and join points identified from the base code.
The mutants are produced more often at higher death rates (because of the increased total number of cell divisions).
Therefore, there are more cell divisions for a larger death rate, and as a consequence, more 1-hit mutants are produced.
Cycling mutants are produced by cycling wild-type cells and they grow according to the same law as the cells producing them.
In addition to being rare and independent from physical positioning, sporulation defects are unlikely to be manifested in heterozygous diploids from which double mutants are produced in SGA.
Therefore, this quantity estimates the number of types in the species richness index that will not go extinct and monotonically approaches the asymptotic species richness as new mutants are produced.
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Mutants were produced by using the QuickChange PCR mutagenesis kit (Stratagene).
13C15N-IgFLNa23 15N-IgFLNa23Na23 mutants were produced in E. Coli as previously described [14].
Mutants were produced by site-directed mutagenesis (Quick-Change, Stratagene, La Jolla, CA) using the pHT315Ab harboring cry1Ab gene.
Site-directed mutants were produced using the Stratagene QuickChange protocol.
The R280K, K120R and K161R mutants were produced using QuikChange Site-directed MutAgilents (AgilenTechnologieseSanta Clara, CA CA, USA).
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