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When increased levels of public goods production increase the population growth rate or carrying capacity, cheating mutants are predicted to be under negative frequency-dependent selection (Ross-Gillespie et al., 2007).
While part of the predicted LBD is deleted in both nhr-64 mutations (Figure 4A), transcripts generated by the deletion mutants are predicted to contain exon 8, which encodes the C-terminal activation domain 2 (AF-2), a region crucial for transcriptional activity [28], [29].
All seven dimer mutants are predicted to have phenotypic effects.
These mutants are predicted to restore activity in the H266 variant (Table 1, "restorative" mutants; Figure 2b).
If Mrg15 inhibits these trans-interactions, like Cap-H2, then Mrg15 mutants are predicted to enhance transvection.
The TNK2D572N, TNK2R849W, TNK2R256H, and DDR1R570Q missense mutants are predicted, by at least two in silico algorithms, to impact the function of the encoded proteins (Table 1).
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There was no MCC value (denoted by -*) for predictions by the Lasso model in this case, as all mutants were predicted to see a decrease in solubility.
Five optimal single mutants were predicted by the computational design, and the N52Y mutation showed the thermostabilization effect.
Seven of the hypothetical mutants were predicted to be missense and were chosen for validation, including one AhLOX7_3' and six AhLOX7_5'.
For the majority of genes (76.1%) covered by both studies, the replication of barcodes in the mixed pool of mutants was predicted by previous data for their targetability (Table S1; Figure 5A).
The inferred timings of the establishment of the two haplotypes are accurate, with the mutant in the red population being predicted to establish after 111 generations (real time 106 generations), and in the blue population mutants being predicted to establish after 94 and 426 generations (real times 83 and 429 generations).
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