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However, embryos 'fathered' by PEL mutants are inviable.
Previous studies also showed that spt10 Δ asf1 Δ double mutants are inviable (Sutton et al. 2001).
We could not perform a similar analysis with RAD27, as rad27 Δ rad52 Δ mutants are inviable (Symington 1998).
When Pol II GOF alleles are combined with the strong upstream shifting allele tfg2 ∆146-180, the double mutants are inviable on this medium.
EGFR null mutants are inviable; however, a temperature sensitive (ts) allele of EGFR enables controlled inactivation of the RTK's activity (Kumar et al., 1998).
Null mutations in MRE11 and RAD50, which encode binding partners of NBS1, are lethal in vertebrates, and mouse Nbs1-null mutants are inviable (Maser et al, 2001).
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In independent work, sly1ts sec18-1 double mutants were inviable (Kosodo et al., 2003).
Conditional mutants in the Saccharomyces cerevisiae cognate were shown to result in increased heat sensitivity, whereas CDC123 null mutants were inviable [ 8].
sua7 -1, sua7 -3, and sua7 -58A5 showed very strong negative interactions with all LOF Pol II mutants tested, with most double mutants being inviable.
Mutations in ELG1 and CTF4 are synthetic lethal (SL), namely, despite the fact that each single-mutant grows well, the double mutant is inviable [2], [4].
These genes were filtered out during analysis because the single mutant was inviable when exposed to 4 µg/ml CPT.
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