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In the first case, faults in P are uncovered when fault-revealing mutants are identified.
Additionally, if gain-of-function mutants are identified in KDM oncoproteins, these may be prime candidates for existing KDM inhibitors or new targeted therapies (Rotili and Mai, 2011).
Once relevant mutants are identified, tagged gene(s) are isolated using a variety of methods.
If deleterious mutants are identified, they can be assigned to a particular genome within bread wheat (A, B or D).
In forward genetic screens in model organisms, genomes are generally randomly mutated by radiation, chemical mutagens or insertional mutagenesis using transposons, after which mutants are identified by phenotypic assay and the phenotype tied back to the gene that has been mutated.
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Recently, several monogenic root-specific mutants were identified in rice (Table 1).
The equivalent mutants were identified manually, and the execution and analysis were performed automatically by scripts.
Lower: 2 mutants were identified in 96 independent lines transformed the target-1 sgRNA:hSpCas9.
Upper: 14 mutants were identified in 90 independent lines transformed the target-1 sgRNA:pSpCas9.
Replacements of amino acid on beneficial mutants were identified as T66L/D70N, T66V/D70N, E83K, E83H and E83N.
For the target-2 sgRNA-pSpCas9 construct, 94 independent transgenic lines were generated, and 2 mutants were identified (2.1% mutated).
More suggestions(15)
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shifts are identified
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mutants are found
mutants are reported
mutants have identified
mutants are rounded
mutants are called
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