MZ yap1 −/− mutants were, however, still lower than double mutants (−27%, p = 1.09E-11).
(F ) Out of plane crossing of C4da neuron dendrites in Ret mutants was however highly elevated (mean ± SD, n = 8; p < 0.001, Student's two-tailed t -test).
The 103+/ kasA mutants were, however, found significantly more frequently in phagolysosomes and this effect could be reversed by expressing the recombinant wild-type kasA gene in the mutant (Fig. 6A).
The increase observed in the mutant was however significantly less than that elicited by the wildtype hOct4 promoter sequence, leading us to conclude that the trans-activation potential of the human Oct4 promoter by B-MYB is elicited, at least in part, through the MYB-binding site located in the proximal promoter of this gene.
This mutant is, however, affected rather in secretion with endocytosis being more or less intact [ 36].
The phosphorylation-deficient receptor mutant was, however, seen to be robustly coupled to Gq/11-protein pathways such as phosphoinositide hydrolysis and calcium signalling.
The period of the double mutant was however intermediate between the single mutants, being slightly reduced compared to the soc1 single mutant (τ = 24.49 ± 0.24 h vs. 24.98 ± 0.22 h, P= 0.048).
In contrast, the pmt1 and pmt4 single mutants are viable; however, the pmt1/pmt4 deletions are synthetically lethal.
We demonstrated that while ΔhrpB2+pUFR047_hrpB21-56, ΔhrpB2+ pUFR047_hrpB21-123 and ΔhrpB2+pUFR047_hrpB2T125A strains are not able to cause citrus canker, the truncated HrpB2XAC polypeptides and HrpB2XAC single amino acid substitution mutants are all however secreted to the extracellular space.
EGFR null mutants are inviable; however, a temperature sensitive (ts) allele of EGFR enables controlled inactivation of the RTK's activity (Kumar et al., 1998).
