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As an increasing fraction of all possible yeast double mutants are grown and tested for genetic interactions, the problem of ascertainment bias in the data with resolve on its own.
However, when fld mutants are grown under carbon limitation conditions, conidiation occurs at 25°C but not 34°C (Springer and Yanofsky 1989).
However, we have found that Snf2 is still recruited to centromeres when conditional Ndc80 mutants are grown at the nonpermissive temperature (Gkikopoulos, data not shown).
After sufficient time for phagolysosome formation to occur, macrophages are homogenized, (phago- lysosomes are collected with a magnet and contained phago- lysosomes are grown on nutrient broth.
In fact, competition environments in which multiple mutants are grown together can be rather different from medium containing only monocultures or two strains.
When pex mutants are grown on oleic acid-containing medium, we indeed observed a rapid loss of viability associated with high FFA levels (Fig. S1).
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Congenic wild-type and pri2 mutants were grown at 23°C to log phase.
(c) Col-0 and AtBS14b mutants were grown under continuous dim light for 7 days and seedlings were photographed.
a WT and the indicated mutants were grown on solid THY (sucrose+) plates with for 24 h, and the colonies were photographed.
a WT and the indicated mutants were grown on solid THY (sucrose+) plates for 24 h, and the colonies were photographed.
(B) Congenic wild-type and pri2 mutants were grown at 23°C to log phase and then shifted to 37°C for an additional 2, 4 and 8 h.
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