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The average GFP fluorescence lifetime of transfected cells with and without cotransfection of Cdc42 mutants are given in table (Fig. 3 (v)).
The model parameter setting for the wildtype simulations as well as for the simulations of mutants are given in Table 1 and Table 2.
The distributions of computed structural branchpoint distances for each of the RPS17B mutants are given in Figure 1.
Procedures, primers and templates used for directed-mutagenesis and resultant mutants are given in Supplementary Material and Table S1.
The base-pair probability values for the wildtype RPS17B intron and all of Libri et al.'s [ 8] mutants are given in Table 3.
A list of all the strains used in this study is provided in supplementary Table 4. Details of the strain construction for specific mutants are given below.
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Similarly in 6B (or 6C), significance at P<0.001 are given, but we cant tell whether LYS1 is different from mutanolysin or whether all the treatments in the mutants are giving the same or different values.
Mutants were given a "strong" (S) classification if the amount of red tissue was substantially less at 30° than at 25°.
Mutagenic primers used to obtain each mutant are given in Table 1.
The average structural branchpoint distances of 1000 suboptimal structures sampled from within 5% of the MFE for each mutant are given in Table 2.
Information on the generation and complementation of a Δafoch4 mutant is given in Supplementary Figure S4.
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